I'm voting for The Joker, because of six reasons...
1. He appeared in the 1940's before the Twisted Metal franchise was even created in November 5, 1995
2. He's been listed among the greatest comic book villains and fictional characters ever created.
3. He's served to be Batman's adversary and arch-enemy!
4. A psychopath with a warped, sadistic sense of humor.
5. HIS INSANE MANIACAL LAUGH!!!!
And 6. He murdered Jason Todd and parylized of one of Batman's allies, Barbara Gordon.