* Matt Pinfield: Villain from the animated Naked Brothers Band special "Supetastic 6" and leader of the Knights of Cerebus. Pilots the Gunman "Lazengann Mk-II"
* Ganondorf: Villain from The Legend of Zelda. 2nd-in-command of the Knights of Cerebus and archenemy of Tad C. DeCartoon
. Pilots the giant robot. A giant talking bee. Pilots the super robot "Nemesis Mazinger"
* Bowser: Villain from Super Mario Bros. and 3rd-in-command of the Knights of Cerebus. Pilots the Mobile Suit "RX-78 3 Nemesis Gundam"
* Wario: Character from Wario Land. Pilots the giant robot "Nemesis Voltes V"
* Dr. V: Leader of the EEVL Society Destler. Her real name is Vanessa Masters. She pilots the giant robot "Nemesis Gaiking"
* Dr. Beeswax: An giant talking bee and Tad DeCartoon's archenemy. Pilots the giant robot "Valsion Mk-II"